Caulobacter crescentus ftsE del
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2016-09-04
Data Taken By: Qing Yao
Species / Specimen: Caulobacter crescentus
Strain: NA1000
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: 12.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Goley ED at Johns Hopkins University School of Medicine
Purification / Growth Conditions / Treatment: When OD600 reaches 0.30, 2 ml medium was spun down gently (3000 rpm for 3 min) and the resuspension was 100 ul for cryo-grid preparation.
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 190/A2. defocus: -12um. magnification: 22500.
Attached Files
square20031.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
square20031_part121_5.rec, Reconstruction (Pixel Size 1.92 nm), 311.8 MB
Files
keyimg_qya2016-09-04-45.jpg
Additional details
Identifiers
- DOI
- 10.22002/cn0wb-11g42
- TILT_SERIES_ID
- qya2016-09-04-45
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-09-04
- Created
-
2016-09-05
- Updated
-
2016-09-06