Myxococcus xanthus PilO-sfGFP

Description

Technical info

Tilt Series Date: 2014-05-13

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.8

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3.5 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.

ycw2014-05-13-9_slicer4942.jpg: Pilli

Attached Files

20140513_YWC101_20002_part131_3.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB

20140513_YWC101_20002.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB