Published 2019 | Version v1
Dataset Open

Caulobacter delta-CpaF

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Tad pilus system

Technical info

Tilt Series Date: 2019-02-15

Data Taken By: Steven Wang

Species / Specimen: Caulobacter crescentus

Strain: AM369, ΔldpF

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 193.8 eV/Ų, defocus: -6.0 μm, magnification: 42000x.

Microscope: Caltech Titan Krios

Acquisition Software: SerialEM

Upload Method: pipeline

Processing Software Used: iMOD

Collaborators and Roles: Patrick Viollier who provided WT and mutants

Purification / Growth Conditions / Treatment: 28 degrees Celsius

Sample Preparation: plunge-freezing

Attached Files

stalk_13.mrc, Tilt Series (Pixel Size 0.351 nm), 3.4 GB

stalk_13.rec, Reconstruction (Pixel Size 1.404 nm), 711.2 MB

Files

keyimg_swy2019-02-15-28.jpg

Files (4.2 GB)

Name Size
md5:eae6fd543c6da1ebbf7ec15ef6fb94f3
992.0 kB Preview Download
md5:61cb7d4ef5378c872aec0de55b51ecbf
13.4 MB Preview Download
3.4 GB (external) Download
711.2 MB (external) Download

Additional details

Identifiers

DOI
10.22002/d20pq-kwc29
TILT_SERIES_ID
swy2019-02-15-28

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2019-02-15
Created
2019-02-18
Updated
2019-02-18