Published 2015 | Version v1
Dataset Open

Vibrio DeltaVC1403

  • 1. ROR icon California Institute of Technology

Contributors

Description

Vibrio cholerae strain CO35 (delta VC1403)cytoplasmic chemoreceptor in chemotaxis operon with CheA1(F9 chemotaxis system)

Technical info

Tilt Series Date: 2015-01-16

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: strain generated by Catherine Oikonomou

Purification / Growth Conditions / Treatment: grown 24 hrs in LB at 30C, diluted 150 µl into 2 ml Ca-HEPES buffer, grown 16 hours at 30C

Attached Files

VibrioCO35setBmanual120014.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibrioCO35setBmanual120014_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

keyimg_aba2015-01-16-15.jpg

Files (4.2 GB)

Name Size
md5:4a12a1c8bd8ad6e120ec04906ad5d6e8
1.1 MB Preview Download
md5:1065d37365c51e45a37195b19bccc67e
23.2 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/d26yw-sj409
TILT_SERIES_ID
aba2015-01-16-15

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-01-16
Created
2015-01-16
Updated
2015-02-25