H. pylori 26695 / AGS

Description

Technical info

Tilt Series Date: 2013-11-25

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 210.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 10% heat inactivated fetal bovine serum at 37 degrees in microaerobic chamber with GasPak EZ Campy Sachets for 2 days, OD600=0.4AGS cells cultivated on QF Au finder grid for 2 day in DMEM + 10% FBS. Infect by pylori for 6 hr.

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated gold (1 ml gold resuspent in 30 ul). Manual blot by Whatman 40 filter paper. Ethane/propane mix.

ycw2013-11-25-53_slicer10092.jpg: Helicobacter pylori . flagellar motor ycw2013-11-25-53_slicer17901.jpg: cag T4SS wi fi particle

Attached Files

20131125_YWC054_10053.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

20131125_YWC054_10053_part121_7.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB