JV9082_K3_janelia

Description

Technical info

Tilt Series Date: 2019-12-05

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3.0°, constant angular increment, dosage: 90.0 eV/Ų, defocus: -4.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: SerialEM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Joseph P. Vogel, Washington University School of Medicine. Data collected and processed by Debnath Ghosal.

Purification / Growth Conditions / Treatment: Cells were grown in AYE liquid culture with following supplements: ferric nitrate, cysteine hydrochloride and thymidine.

Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Data collected at Janelia Krios (K3 camera). Mag: 26000, pixel size: 2.61A. Defocus range -2 to -4 um.

Attached Files

9082_Box2_grid3_40.mrc, Tilt Series (Pixel Size 0.261 nm), 1.6 GB

9082_Box2_grid3_40_full.rec, Reconstruction (Pixel Size 1.044 nm), 737.3 MB