Hyphomonas "SW57"
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-02-25
Data Taken By: Yi-Wei Chang
Species / Specimen: Hyphomonas neptunium
Strain: ATCC 15444
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown overnight in Marine Broth (MB) medium at 30 degrees. 1:20 diluted into GAMS-P medium and incubated for 48 hr at 30 degrees. 10 ml cells were pelleted and resuspended into 10 ml MB, incubate for 5 hr at 30 degrees. Further concentration was made by pelleted down whole culture solution and resuspended into 50 ul fresh MB.
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nM BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix.
Attached Files
20120225_20018.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
20120225_20018_part.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_ycw2012-02-25-25.jpg
Additional details
Identifiers
- DOI
- 10.22002/dhprs-8pc39
- TILT_SERIES_ID
- ycw2012-02-25-25
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-02-25
- Created
-
2012-02-26
- Updated
-
2012-03-12