Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

LBculture_wt

Creators

  • 1. ROR icon California Institute of Technology

Description

This is a LB culture of wt from a flask grown into stationary phase. The purpose was to to check that there are no nanowires and nothing was seen in sem, and when we do cryo-em to ensure we see flagellum. This would ensure that flagellum can't be seen in sem. But we did see long wires in sem. Sahand opined that is because we grew them into stationary phase. I imaged these to capture nanowires since they were present in sem

Technical info

Tilt Series Date: 2015-07-25

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand

Purification / Growth Conditions / Treatment: Manual blotting Cu side glow discharged. Sample was put on opposite side and blotted. Similar to vitrobot

Attached Files

lbculture_nanowirepresent_atlas10001.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB

lbculture_nanowirepresent_atlas10001_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB

Files

keyimg_psa2015-07-25-2.jpg

Files (2.2 GB)

Name Size
md5:d14fb04193da2bb4e90b2dad3cfd034b
1.1 MB Preview Download
md5:3d798e98f7e03a19672f6c0e76b3ae47
15.0 MB Preview Download
1.7 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/djvr9-nc615
TILT_SERIES_ID
psa2015-07-25-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-07-25
Created
2015-07-25
Updated
2015-08-19