Published 2015 | Version v1
Dataset Open

Mycobacterium marinum

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Grown on 2x LB to induce sporulation

Technical info

Tilt Series Date: 2015-10-13

Data Taken By: Elitza Tocheva

Species / Specimen: Mycobacterium marinum

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Will DePas from Dianne Newman's lab grew the cells

Sample Preparation: Cells were frozen with Vitrobot Mark 4. Grids were positively glow discharged.

Attached Files

Mmarinum2_oct20150007_part121_11.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

Mmarinum2_oct20150007.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

Files

keyimg_etl2015-10-13-17.jpg

Files (4.0 GB)

Name Size
md5:866606a06ce47bad70c079259f6a577a
1.1 MB Preview Download
md5:299cbb0c1c88134fff2f388c75416851
15.6 MB Preview Download
3.4 GB (external) Download
534.5 MB (external) Download

Additional details

Identifiers

DOI
10.22002/dpt9e-wj789
TILT_SERIES_ID
etl2015-10-13-17

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-10-13
Created
2015-10-13
Updated
2019-02-14