Published 2011 | Version v1
Dataset Open

E. coli (pLE6, 20 um IPTG)

Creators

  • 1. ROR icon California Institute of Technology

Description

e. coli from Larry Rothfield containing plasmid carrying WT MreB.

Technical info

Tilt Series Date: 2011-07-29

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: Leginon

Upload Method: pipeline

Processing Software Used: raptor

Sample Preparation: Induced with 20um IPTG for 2 hours starting at OD600=0.2.

mst2011-07-29-17_slicer4971.jpg: Unknown

Attached Files

20110729_ple6_20umIPTG0010_full.rec, Reconstruction (Pixel Size 1.892 nm), 1.0 GB

20110729_ple6_20umIPTG0010.mrc, Tilt Series (Pixel Size 0.946 nm), 1.0 GB

Files

keyimg_mst2011-07-29-17.jpg

Files (2.1 GB)

Name Size
1.0 GB (external) Download
1.0 GB (external) Download
md5:73a72efa202e2655b6b31de7032a5314
1.2 MB Preview Download
md5:9da68e0d92ee7e5da988b10e82f7a922
33.0 MB Preview Download
md5:8bca61ab69763dcbfb8f1fb42bdb3210
344.1 kB Preview Download

Additional details

Identifiers

DOI
10.22002/drzch-68z19
TILT_SERIES_ID
mst2011-07-29-17

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-07-29
Created
2014-07-01
Updated
2014-07-07