COS7 pNLDN
Description
Technical info
Tilt Series Date: 2013-08-18
Data Taken By: Cora Woodward
Species / Specimen: Cos7
Strain: Cos7 cells
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSF tomo
Collaborators and Roles: pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells were grown on grids and transfected using lipofectamine
Sample Preparation: manual blotting
Attached Files
13aug18_C125_2_COS7pNLDN_AT1BT1_0005.mrc, Tilt Series (Pixel Size 0.5 nm), 2.3 GB
13aug18_C125_2_COS7pNLDN_AT1BT1_0005_full.rec, Reconstruction (Pixel Size 2.0 nm), 534.5 MB
Files
keyimg_cwl2013-08-18-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/dtq2e-vth38
- TILT_SERIES_ID
- cwl2013-08-18-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-08-18
- Created
-
2013-08-18
- Updated
-
2019-03-06