Published 2009 | Version v1
Dataset Open

GFP-MreB fusion methanol control

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Raw data files of GFP-MreB fusion methanol control

Technical info

Tilt Series Date: 2009-08-01

Data Taken By: Zhuo Li

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -12.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: RAPTOR, REACH

Collaborators and Roles: Experiments were done by Zhuo Li.

Purification / Growth Conditions / Treatment: The GFP-MreB-expressing NA1000 cells were cultured to exponential phase and treated with methanol as a control.

Sample Preparation: 10 nm gold.

Attached Files

09aug01a_methanolgfmr1_059.mrc, Tilt Series (Pixel Size 1.2 nm), 1.0 GB

09aug01a_methanolgfmr1_059_full.rec, Reconstruction (Pixel Size 2.4 nm), 367.0 MB

Files

keyimg_zla2009-08-01-59.jpg

Files (1.4 GB)

Name Size
1.0 GB (external) Download
367.0 MB (external) Download
md5:2706a0f0984d43b9125235dc7fdce8ce
1.2 MB Preview Download
md5:f3fc58f69d77dcb0041c2ff320ddce80
10.4 MB Preview Download

Additional details

Identifiers

DOI
10.22002/dxt06-ae022
TILT_SERIES_ID
zla2009-08-01-59

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-08-01
Created
2009-08-04
Updated
2009-08-11