CLEM of TRIM5a-YFP in HeLa cells
Description
Technical info
Tilt Series Date: 2014-06-17
Data Taken By: Stephen Carter
Species / Specimen: Hela cells expressing TRIM5a-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 136.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: Undefined
Upload Method: reupload_downloaded
Processing Software Used: IMOD and Raptor
Collaborators and Roles: Tom Hope and Wes Sundquist
Purification / Growth Conditions / Treatment: Gold London finder grids glow discharged twice. Concentration of red 200 nm beads 1/300 on grid. 10 nm gold. Manual blotting. Cells grown on grids for 2 days and look vesiculated.
Attached Files
tomography5.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
tomography5_full.rec, Reconstruction (Pixel Size 2.4 nm), 445.4 MB
tomography5.rec, Reconstruction (Pixel Size 0.0 nm), 421.4 MB
Files
keyimg_scd2014-06-17-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/dz0yn-6bs23
- TILT_SERIES_ID
- scd2014-06-17-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-06-17
- Created
-
2014-06-25
- Updated
-
2015-05-05