Published 2011
| Version v1
Dataset
Open
M222R CheA inactive
Contributors
Researcher:
Description
Chemoreceptor mutant, receptors locked in an CheA inactive state
Technical info
Tilt Series Date: 2011-11-28
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: strain and plasmid from Sandy Parkinson
Purification / Growth Conditions / Treatment: grown in TB medium with Chloramphenicol overnight at 30 degrees, then lysed with Penicillin for an hour
Attached Files
strainM222Rday1_0005.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
strainM222Rday1_0005_part.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_aba2011-11-28-58.jpg
Additional details
Identifiers
- DOI
- 10.22002/dzemf-71m97
- TILT_SERIES_ID
- aba2011-11-28-58
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-11-28
- Created
-
2011-11-30
- Updated
-
2011-12-06