Sulfolobus cells synchronized fo
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2014-02-04
Data Taken By: Rasika Ramdasi
Species / Specimen: Sulfolobus acidocaldarius
Strain:
Tilt Series Settings: Single Axis, tilt range: (-55.0°, 55.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Megan Dobro's samples gridbox 8.
Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine
Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 5 sec, sponge for humidity
Attached Files
atlas30007.mrc, Tilt Series (Pixel Size 0.39 nm), 3.2 GB
atlas30007_part111_6.rec, Reconstruction (Pixel Size 1.56 nm), 623.6 MB
Files
keyimg_rra2014-02-04-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/e0yng-dmg62
- TILT_SERIES_ID
- rra2014-02-04-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-02-04
- Created
-
2014-02-04
- Updated
-
2014-02-07