E. coli mini cell WM3433

Description

Technical info

Tilt Series Date: 2013-09-10

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2.0°, constant angular increment, dosage: 20.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Janelia Titan

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid

ycw2013-09-10-34_slicer6958.jpg: Peptidoglycan ycw2013-09-10-34_slicer6960.jpg: pilli ycw2013-09-10-34_slicer6961.jpg: pilli

Attached Files

20130910_Janelia_Bdellovibrio_WM3433_20005.mrc, Tilt Series (Pixel Size 0.42 nm), 1.4 GB

20130910_Janelia_Bdellovibrio_WM3433_20005_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB