CLEM of TRIM5a-YFP in HeLa cells
Description
Technical info
Tilt Series Date: 2014-07-09
Data Taken By: Stephen Carter
Species / Specimen: Hela cells expressing TRIM5a-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 153.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: autoPatch
Collaborators and Roles: Tom Hope and Wes Sundquist
Purification / Growth Conditions / Treatment: Gold London finder grids glow discharged twice. Concentration of red 200 nm beads 1/300 on grid. 10 nm gold. Manual blotting. Cells grown on grids for 2 days and look vesiculated.
Attached Files
tomography1.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
tomography1.rec, Reconstruction (Pixel Size 1.56 nm), 1.8 GB
Files
keyimg_scd2014-07-09-1.jpg
Additional details
Identifiers
- DOI
- 10.22002/e28st-bw386
- TILT_SERIES_ID
- scd2014-07-09-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-07-09
- Created
-
2014-07-09
- Updated
-
2015-01-31