Published 2013 | Version v1
Dataset Open

HUVECs infx pNLGFP

  • 1. ROR icon California Institute of Technology

Description

pNLGFP obtained from Irvin Chen (UCLA)

Technical info

Tilt Series Date: 2013-10-22

Data Taken By: Cora Woodward

Species / Specimen: HUVECs

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: pNLGFP obtained from Irvin Chen (UCLA)

Purification / Growth Conditions / Treatment: Whole cells were grown on Au finder grids and infected with pNLGFP- VSVG. Sample frozen 36h post infection

Sample Preparation: manual blotting

Attached Files

C169-2_HUVECs_pNL36h_atlas3_0003.mrc, Tilt Series (Pixel Size 0.42 nm), 3.3 GB

C169-2_HUVECs_pNL36h_atlas3_0003_part121_2.rec, Reconstruction (Pixel Size 1.68 nm), 516.7 MB

Files

keyimg_cwl2013-10-22-4.jpg

Files (3.9 GB)

Name Size
3.3 GB (external) Download
516.7 MB (external) Download
md5:3af9a8c2fb4dbc6f24bb0776ac53810b
1.1 MB Preview Download
md5:5a87d69031a6d27724a6ef5890b723cd
21.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/e2ffq-v1a28
TILT_SERIES_ID
cwl2013-10-22-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2013-10-22
Created
2013-10-29
Updated
2019-03-07