Hippocampus Neurons

Description

Technical info

Tilt Series Date: 2020-02-16

Data Taken By: Stephen Carter

Species / Specimen: Rat Hippocampal Neuron (cultured)

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -3.0 μm, magnification: 33000x.

Acquisition Software: SerialEM

Upload Method: pipeline

Processing Software Used: IMOD

Collaborators and Roles: Zach Freyberg

Purification / Growth Conditions / Treatment: ssociated rat cortical and hippocampal neurons were prepared from embryonic day 18 (E18) Long-Evans rats (Charles River Laboratories, Wilmington, MA). Neurons were plated onto 35-mm- diameter circular glass-bottom dishes (MatTek, Ashland, MA) coated with poly-D-lysine (20 mg/ml) and laminin (3.4 mg/ml) at a density of 3.25 × 105 cells/ml (total volume, 2 ml). The neurons were maintained in neurobasal medium supplemented with B27, penicillin/streptomycin (100 U/ml and 100 mg/ml, respectively), and 2 mM glutamine. One-fifth of the medium in each dish was replaced every 4 days.

Sample Preparation: Manual Blotting

Attached Files

neuron2.mrc, Tilt Series (Pixel Size 2.65 nm), 2.9 GB

neuron2_full.rec, Reconstruction (Pixel Size 10.6 nm), 1.5 GB