Saccharomyces cerevisiae - JAWY1
Description
Technical info
Tilt Series Date: 2016-11-30
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.
Purification / Growth Conditions / Treatment: Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. sm2016-11-30-1to5,*-10,*-13to20 (TS1-TS14)- Heat shocked @38 deg C for 2min10min. sm2016-11-30-6to9,*-11to12 (TS15-21) - Heat shocked @38 deg C for 1.5 hr and recovered @28deg C for 40 min.
Attached Files
TS11.mrc, Tilt Series (Pixel Size 0.39 nm), 1.5 GB
TS11_DB.rec, Reconstruction (Pixel Size 0.0 nm), 313.6 MB
Files
keyimg_smk2016-11-30-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/e8awk-1b684
- TILT_SERIES_ID
- smk2016-11-30-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-11-30
- Created
-
2017-01-12
- Updated
-
2017-01-18