Published 2009 | Version v1
Dataset Open

Hyphomonas EB session17 27.5KX

Creators

  • 1. ROR icon California Institute of Technology

Description

high-order DNA, H-NS, nucleoid, asymmetric cell division

Technical info

Tilt Series Date: 2009-06-07

Data Taken By: Jian Shi

Species / Specimen: Hyphomonas neptunium

Strain: ATCC 15444

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: imod

Collaborators and Roles: Cultured by Jian; data collected by Jian; processed by Jian

Purification / Growth Conditions / Treatment: exponential phase in PYE media, EB 0.01mg overnight at 4degree...

Sample Preparation: QF grid, 10nm gold, 100% humidity...

jsa2009-06-07-16_slicer8826.jpg: Hyphomonas neptunium . Nucleoid EtBr jsa2009-06-07-16_slicer8827.jpg: Hyphomonas neptunium . Unknown ring

Attached Files

09jun07a_bx675a_017.mrc, Tilt Series (Pixel Size 0.774 nm), 1.0 GB

09jun07a_bx675a_017_1k.rec, Reconstruction (Pixel Size 1.548 nm), 218.1 MB

Files

jsa2009-06-07-16_slicer8826.jpg

Files (1.2 GB)

Name Size
1.0 GB (external) Download
218.1 MB (external) Download
md5:e8dec0b02a201a17f1a9e269e1f4caae
197.4 kB Preview Download
md5:f37c03d78adf577670618bf903216478
149.1 kB Preview Download
md5:cc774a7e44d929b797fb787b3e5b68ff
966.5 kB Preview Download
md5:d3f7682082d3297cba688b0d17cf6c46
5.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/edbzv-ndh97
TILT_SERIES_ID
jsa2009-06-07-16

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-06-07
Created
2009-10-20
Updated
2014-07-29