Single cell RNA sequencing paradigm after chronic loss of inhibition in a pre-motor avian circuit

Description

Mastering essential motor skills is crucial for an animal's survival and reproduction, enabling it to endure diverse perturbations throughout its lifespan, such as trauma, disease, and aging. What mechanisms orchestrate brain circuit reorganization and recovery to maintain stability of behavioral output during the continued presence of a disturbance? In this study, we investigate the consequences at the behavioral, neuronal, and transcriptome level of chronically perturbing a complex behavior over time. Our electrophysiology recordings suggest a relationship between offline activity dynamics and circuit restoration for behavior. Furthermore, single-cell RNA sequencing reveals cellular mechanisms underlying behavioral recovery suggesting a role for synaptic plasticity in this restoration process mediated by microglia and MHC class I. Our findings offer compelling evidence that the adult brain has the ability to surmount prolonged circuit perturbations through the utilization of offline activity and repair mediated by microglia.

This dataset contains the single-cell RNA sequencing files in two control and two experimental animals ( after chronic muting of interneurons) in the avian pre-motor circuit. This is the first dataset of its kind where a behavioral manipulation leading to drastic changes is compared against a control in the zebra finch HVC. 

Please contact us with any questions, comments or concerns: szofitorok8@gmail.com , zsofi@caltech.edu, lauraluebbert@caltech.edu, clois@caltech.edu

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Technical info

Due to lack of space the missing file for SE7425 SA112356 S1 L003 R1 001 can be found with our other dataset with DOI: https://doi.org/10.22002/3ta8v-gj982 .