Thermococcus koakarensis

Description

Technical info

Tilt Series Date: 2016-08-25

Data Taken By: Debnath Ghosal

Species / Specimen: Thermococcus kodakarensis

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain sent to us by Robert Gunsalus, UCLA. Data collected and processed by Kiwoo and Debnath Ghosal

Purification / Growth Conditions / Treatment: Standard Tk medium with starch and sulfur

Sample Preparation: 8 grids were frozen for the this strain. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 3.5 sec. Wait time 8 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

Attached Files

Tk_250820160020.mrc, Tilt Series (Pixel Size 0.39 nm), 2.3 GB

Tk_250820160020_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB