Published 2013 | Version v1
Dataset Open

Bdellovibrio/E. coli minicell

Chang, Yi-Wei1
  • 1. ROR icon California Institute of Technology

Description

Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion

Files

keyimg_ycw2013-09-28-46.jpg
Files (28.7 MB)
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md5:506790cac54746134cc8b75b0929e41d
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Technical info

Tilt Series Date: 2013-09-28

Data Taken By: Yi-Wei Chang

Species / Specimen: Bdellovibrio bacteriovorus

Strain: HD100

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg

Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min.

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder

ycw2013-09-28-46_slicer14254.jpg: Bdellovibrio bacteriovorus . flagelar motor arrows at c-ring? . flagellum

External Files

Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-46

20130928_YWC034_10046.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB Download

20130928_YWC034_10046_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB Download

Additional details

Created:
May 24, 2023
Modified:
May 24, 2023