Published 2013
| Version v1
Dataset
Open
Bdellovibrio/E. coli minicell
- Researchers:
- Sockett, Liz
- Liu, Jun
- Margolin, William
- Rettberg, Lee
Citation
APA
Chang, Y.-W. (2013). Bdellovibrio/E. coli minicell [Data set]. CaltechDATA. https://doi.org/10.22002/efz81-5ft44
Description
Bdellovibrio invasion complex with E. coli WM3433 minicell, early stage of invasion
Files
keyimg_ycw2013-09-28-46.jpg
Technical info
Tilt Series Date: 2013-09-28
Data Taken By: Yi-Wei Chang Species / Specimen: Bdellovibrio bacteriovorus Strain: HD100 Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Bdellovibrio Strain from Liz Sockett, WM3433 E. coli minicell from Jun Liu & William Margolin, Sample prepared by Lee Rettberg Purification / Growth Conditions / Treatment: Bdellovibrio cells grown in S17-1 prey solution at 30 degrees, then filtered by 0.45 um filter, concentrated by centrifugation. WM3433 E. coli minicells were enriched by 2 step centrifugation (3,000 rcf, 3 min --> 9,000 rcf, 5 min). Invasion complex were made by mixing them for 15 min. Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu finder ycw2013-09-28-46_slicer14254.jpg: Bdellovibrio bacteriovorus . flagelar motor arrows at c-ring? . flagellumExternal Files
Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2013-09-28-46
20130928_YWC034_10046.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB Download 20130928_YWC034_10046_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB DownloadAdditional details
- TILT_SERIES_ID
- ycw2013-09-28-46
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2013-09-28
- Created
-
2013-09-30
- Updated
-
2015-08-19