Published 2017 | Version v1
Dataset Open

V.cholerae

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.

Technical info

Tilt Series Date: 2017-01-06

Data Taken By: Poorna Subramanian

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Yi-wei

Purification / Growth Conditions / Treatment: growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume

Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.

psa2017-01-06-91_slicer17396.jpg: chemoreceptor arrays psa2017-01-06-91_slicer17397.jpg: IM bleb decorated? psa2017-01-06-91_slicer17398.jpg: IM bleb decorated?

Attached Files

sq90010.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

sq90010_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB

Files

keyimg_psa2017-01-06-91.jpg

Files (4.2 GB)

Name Size
md5:88be8224fa4495cc4521a0237344d904
1.1 MB Preview Download
md5:5e7bbff4bf530ea33f4c71484169c236
23.9 MB Preview Download
md5:beeb94556289d36a22542b976cbe0de0
413.5 kB Preview Download
md5:0a98d3af17a4c1febf83bb011480f433
620.1 kB Preview Download
md5:8266034741587bc78293368234881d7f
594.2 kB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/egqfj-3xt57
TILT_SERIES_ID
psa2017-01-06-91

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-01-06
Created
2017-01-09
Updated
2017-01-19