Extended Data Figure: Morphology of amphid and phasmid neurons in IFT-20-deficient worms

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Extended Data, Figure 1: (A) Illustration of amphid and phasmid neurons in C. elegans. Cilia are marked at the dendritic tips of these neurons. (B) Examples of the Oil lipophilic dye filling test in sensory amphid neurons of wild-type and ift-20 mutant worms. 100% of the ift-20 null worms fail to incorporate dye (N2::15). (C, D) Examples of the amphid and phasmid sensory neuron morphology in wild-type and ift-20 mutant animals with (C) soluble/cytoplasmic GFP (sGFP) or (D) endogenously labeled IFT-74::GFP. White arrows point at dendritic ends where cilia assemble. Despite having relatively normal length and morphology, the dendrites of ift-20 null animals have enlarged regions towards their tips that likely correspond to accumulations of ciliary proteins (such as IFT-74) that fail to reach the ciliary base (representative examples; N2::15). Scale bars, 10 µm.

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Related Publication: The IFT20 homolog in Caenorhabditis elegans is required for ciliogenesis and cilia-mediated behavior Tiago J Dantas Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal microPublication Biology https://doi.org/10.17912/micropub.biology.000396 eng

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Contact person: Tiago J Dantas tiago.dantas@i3s.up.pt

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