Published 2017
| Version v1
Dataset
Open
Hela-cryoFIB
Description
Raw data files of Hela-cryoFIB
Technical info
Tilt Series Date: 2017-03-27
Data Taken By: Wei Zhao
Species / Specimen: HeLa cells
Strain: ATCC
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Purification / Growth Conditions / Treatment: Hela cells grown on Au finder grids for 28-48 hours.Grids coated with PL.
Sample Preparation: Sample Preparation: Cells were plunge frozen using the vitrobot III blotted manually from back(drain time 1s).
Attached Files
TS8.mrc, Tilt Series (Pixel Size 0.372 nm), 1.6 GB
TS10_bin2.rec, Reconstruction (Pixel Size 0.0 nm), 224.5 MB
Files
keyimg_wza2017-03-27-10.jpg
Additional details
Identifiers
- DOI
- 10.22002/ek227-q5r90
- TILT_SERIES_ID
- wza2017-03-27-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-03-27
- Created
-
2018-06-21
- Updated
-
2018-06-21