Published 2012 | Version v1
Dataset Open

TRIM5-21R 2D lattice

  • 1. ROR icon California Institute of Technology

Description

TRIM5-21R 2D lattice prepared by Barbie Ganser-Pornillos and Viswanathan Chandrasekaran

Technical info

Tilt Series Date: 2012-11-20

Data Taken By: Cora Woodward

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: None μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Viswanathan Chandrasekarangrids purified the protein, Barbie Ganser-Pornillos assembled protein into 2D lattice. The sample was was plunge frozen and imaged at Caltech by Cora Woodward and Kelly Dryden

Sample Preparation: manual blotting

Attached Files

12nov20_TRIM5_21R_grid4_AT1BT1_00010009.mrc, Tilt Series (Pixel Size 0.45 nm), 3.4 GB

12nov20_TRIM5_21R_grid4_AT1BT1_00010009_full.rec, Reconstruction (Pixel Size 1.8 nm), 530.0 MB

Files

keyimg_cwl2012-11-20-13.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
530.0 MB (external) Download
md5:fd00d4d3204673e443ce8f8667641677
1.1 MB Preview Download
md5:1e6f158b68028cf3b0d1564a17561e86
20.7 MB Preview Download

Additional details

Identifiers

DOI
10.22002/eqs8m-a9571
TILT_SERIES_ID
cwl2012-11-20-13

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-11-20
Created
2012-11-21
Updated
2019-03-07