Hylemonella gracilis
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2006-08-16
Data Taken By: Gavin Murphy
Species / Specimen: Hylemonella gracilis
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.1°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod, bsoft
Collaborators and Roles: Cells provided by Jared Leadbetter; Data collected and processed by Gavin Murphy
Purification / Growth Conditions / Treatment: Grown in dilute media without salt. 0.5 g/L of Tryptone, 0.5 g/L YE. Cells grown for 2 days. OD never gets above 0.005. Centrifuged lightly, then concentrated 100x fold.
Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...
Attached Files
S15_S474G3_90e10d22k.mrc, Tilt Series (Pixel Size 0.98 nm), 956.4 MB
S15s.mrc, Reconstruction (Pixel Size 0.0 nm), 175.3 MB
Files
keyimg_gme2006-08-16-14.jpg
Additional details
Identifiers
- DOI
- 10.22002/evfhe-3jw09
- TILT_SERIES_ID
- gme2006-08-16-14
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2006-08-16
- Created
-
2008-06-18
- Updated
-
2008-06-11