COS7 VPS4DN+pNLGFP
Description
Technical info
Tilt Series Date: 2012-11-29
Data Taken By: Cora Woodward
Species / Specimen: Cercopithecus aethiops (monkey, African green)
Strain: COS-7 ATCC CRL-1651
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: None μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: UCSFTomo
Collaborators and Roles: VPS4DN construct provided by Wes Sundquist. pNLGFP construct provided by Irvin Chen, UCLA. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine
Sample Preparation: manual blotting
Attached Files
12nov29_C129_1_pnlDN_AT2BT1_0004.mrc, Tilt Series (Pixel Size 0.49 nm), 3.4 GB
12nov29_C129_1_pnlDN_AT2BT1_0004_part122_13.rec, Reconstruction (Pixel Size 1.96 nm), 530.0 MB
Files
keyimg_cwl2012-11-29-11.jpg
Additional details
Identifiers
- DOI
- 10.22002/ewa51-1zw22
- TILT_SERIES_ID
- cwl2012-11-29-11
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-11-29
- Created
-
2012-11-29
- Updated
-
2019-03-07