Pseudomonas aeruginoas_JT2944
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2018-08-16
Data Taken By: Mohammed Kaplan
Species / Specimen: Pseudomonas aeruginosa
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: RAPTOR
Collaborators and Roles: Cells produced by Jonida Toska from Melakanos lab.
Purification / Growth Conditions / Treatment: The cells were grown overnight in LB at 37 C.
Sample Preparation: Cells from overnight culture were added to thick carbon grids and blotted using the vitrobot in BI. Blot force 6, blot time 3 s, drain time 1s.
Attached Files
PseudoJT2944Tilt17.mrc, Tilt Series (Pixel Size 0.39 nm), 28.4 MB
Files
keyimg_mka2018-08-16-41.jpg
Additional details
Identifiers
- DOI
- 10.22002/f77eg-qgj88
- TILT_SERIES_ID
- mka2018-08-16-41
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-08-16
- Created
-
2018-08-17
- Updated
-
2018-10-01