Saccharomyces cerevisiae - 6210

Description

Technical info

Tilt Series Date: 2017-05-17

Data Taken By: Shrawan kumar Mageswaran

Species / Specimen: Unspecified

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: SerialEM

Upload Method: reupload_downloaded

Processing Software Used: IMOD

Collaborators and Roles: Obtained from Dr. Markus Babst, University of Utah

Purification / Growth Conditions / Treatment: Cells were grown overnight in YPD, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6.

Sample Preparation: Cells were diluted to an OD of 0.2-0.25 in YPD, applied to the grid, blotted from behind manually and plunge frozen in liquid ethane-propane mix. ~200 nm lamellae were FIB milled before imaging by TEM.

Attached Files

TS13.mrc, Tilt Series (Pixel Size 0.39 nm), 1.5 GB

TS13_4bin_DB.rec, Reconstruction (Pixel Size 0.0 nm), 430.3 MB