H. pylori 26695 WT / AGS

Description

Technical info

Tilt Series Date: 2016-11-02

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain and sample prepared by Carrie Shaffer

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ml gold+ 25 ml 10% BSA x4, resuspend in 30 ul). Blot for 5 sec (1 sec longer than usual to remove more solution). Ethane/propane mix.

ycw2016-11-02-8_slicer17150.jpg: cag T4SS tubes? ycw2016-11-02-8_slicer17151.jpg: tube periodicity (pink) and cross sections (green) ycw2016-11-02-8_slicer17152.jpg: tube cross sections ycw2016-11-02-8_slicer17153.jpg: long tube on top of H. pylori cell ycw2016-11-02-8_slicer17871.jpg: cag T4SS wi fi particle?

Attached Files

20161102_YWC229_20006_full.rec, Reconstruction (Pixel Size 1.92 nm), 534.5 MB

20161102_YWC229_20006.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB