Published 2014 | Version v1
Dataset Open

Vibrio deltaCheA2

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

V. cholerae strain deleted for CheA2

Technical info

Tilt Series Date: 2014-04-10

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Ikuro Kawagishi

Purification / Growth Conditions / Treatment: Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C

aba2014-04-10-9_slicer3823.jpg: short polar array, flagellar motor in delta CheA2 strain

Attached Files

VibrioDeltaA20007.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibrioDeltaA20007_part121_5.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

aba2014-04-10-9_slicer3823.jpg

Files (4.2 GB)

Name Size
md5:2c77d4644480e6d75cc6202045160fdf
327.8 kB Preview Download
md5:0e0b0e7bf52dc479209ebb8ca190f717
1.1 MB Preview Download
md5:5418710a3faea5d76550e4f3d656e57d
26.7 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/fgc8p-kqn14
TILT_SERIES_ID
aba2014-04-10-9

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-04-10
Created
2014-04-10
Updated
2014-09-24