Helicobacter pylori 26695
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-10-09
Data Taken By: Yi-Wei Chang
Species / Specimen: Helicobacter pylori
Strain: 26695
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Cell prepared by Lee Rettberg.
Purification / Growth Conditions / Treatment: Cells grown in Brucella broth (Fluka) + 10% heat inactivated fetal bovine serum at 37 degrees + 5% CO2, OD600=0.4
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: QF Cu R2/2 200
ycw2013-10-09-13_slicer10227.jpg: Helicobacter pylori . chemoreceptor . Ribosome Exclusion Zone . REZ
Attached Files
20131009_Hpylori_26695_BB_10013.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB
20131009_Hpylori_26695_BB_10013_part131_2.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2013-10-09-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/fhqpr-9rz70
- TILT_SERIES_ID
- ycw2013-10-09-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-10-09
- Created
-
2013-10-10
- Updated
-
2014-08-12