E.coli "strain 3" G235E CheA on
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-02-10
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Sandy Parkinson
Purification / Growth Conditions / Treatment: culture grown in TB medium (+GEntamycin) to OD (600) 0.31 at 30 degrees. Additional one hour incubation with penicillin before freezing.
Sample Preparation: freezing with Vitrobot @ 100 % humidity, -4 offset, 3.5 s blot time, no drain, 3 µl sample, pure ethane.
Attached Files
strain30022.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
strain30022_full.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_aba2012-02-10-22.jpg
Additional details
Identifiers
- DOI
- 10.22002/fj2wz-zhn66
- TILT_SERIES_ID
- aba2012-02-10-22
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-02-10
- Created
-
2012-02-11
- Updated
-
2012-02-16