Published 2016
| Version v1
Dataset
Open
Cell line25 incubated MG-132
Contributors
Researcher:
Description
4 hours incubation MG-132 (0.4 ul) 1 μg/ml. Added 500 ul of Vpr-tomato pseudovirus to each well.
Technical info
Tilt Series Date: 2016-02-29
Data Taken By: Stephen Carter
Species / Specimen: Hela cells expressing TRIM5a-YFP
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: IMOD
Collaborators and Roles: Tom Hope
Purification / Growth Conditions / Treatment: Grown on R2/2 London finder grids coated with fibronectin
Sample Preparation: Manual blotting
Attached Files
tomography4.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB
tomography4_full.rec, Reconstruction (Pixel Size 2.4 nm), 890.9 MB
Files
keyimg_scd2016-02-29-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/fpcsw-9gf02
- TILT_SERIES_ID
- scd2016-02-29-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-02-29
- Created
-
2017-12-15
- Updated
-
2017-12-21