Crosslinked HIV-1 cores TRIM5α
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-01-03
Data Taken By: Stephen Carter
Species / Specimen: HIV cores
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 84.0 eV/Ų, defocus: -8.0 μm, magnification: 41000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: IMOD and Raptor
Collaborators and Roles: Wes Sundquist
Purification / Growth Conditions / Treatment: the TRIM5α-core complexes will be purified through a sucrose cushion to remove excess unbound TRIM5alpha before crosslinking and freezing
Sample Preparation: Non infectious HIV-1 cores decorated with TRIM5α. 0.3 µM sulfo-EGS
Attached Files
tomography0009.mrc, Tilt Series (Pixel Size 0.26 nm), 3.4 GB
tomography0009_full.rec, Reconstruction (Pixel Size 1.04 nm), 445.4 MB
Files
keyimg_scd2015-01-03-53.jpg
Additional details
Identifiers
- DOI
- 10.22002/fq38b-wsx06
- TILT_SERIES_ID
- scd2015-01-03-53
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-01-03
- Created
-
2015-01-03
- Updated
-
2016-03-01