Rhodobacter sphaeroides JPA 1558
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-06-03
Data Taken By: Ariane Briegel
Species / Specimen: Rhodobacter sphaeroides
Strain: ATCC 17023
Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 2°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 33000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Judy Armitage
Purification / Growth Conditions / Treatment: grow (1 day) in SUX medium + Naldixic acid (6.25 µl of 20mg/ml stock) + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*Spin down 5ml per strain, resuspend pellets (barely visible) in SUX medium + Kanamycin (2.5 µl stock 1000x) + 25 µl 10mM IPTG.*grow overnight.
Sample Preparation: cryosectioning by MArk LAdinsky
Attached Files
atlas_grid-2_7x7cryosections0046.mrc, Tilt Series (Pixel Size 0.38 nm), 1.8 GB
atlas_grid-2_7x7cryosections0046_part67_14.rec, Reconstruction (Pixel Size 0.76 nm), 3.4 GB
Files
keyimg_aba2013-06-03-42.jpg
Additional details
Identifiers
- DOI
- 10.22002/fsmkg-ev981
- TILT_SERIES_ID
- aba2013-06-03-42
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-06-03
- Created
-
2013-06-03
- Updated
-
2013-07-18