Published 2017
| Version v1
Dataset
Open
U2OS-Tubulin Tracker Green
Description
U2OS cells stained with tubulin tracker to locate midbodies. Cryo-FIB milling is used to make the sample thinner.
Technical info
Tilt Series Date: 2017-06-13
Data Taken By: Wei Zhao
Species / Specimen: U2OS
Strain:
Tilt Series Settings: Single Axis, tilt range: (-64.0°, 64.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: pipeline
Processing Software Used: IMOD-patch tracking
Purification / Growth Conditions / Treatment: U2OS cells grown on Au finder grids for 28-48 hours.Grids coated with PL.
Sample Preparation: Cells were stained with tubulin tracker and Hoechst before freezing. Plunge frozen using the vitrobot III blotted manually from back(drain time 1s).
Attached Files
TS1_7A.mrc, Tilt Series (Pixel Size 0.371 nm), 910.8 MB
Files
keyimg_wza2017-06-13-1.jpg
Additional details
Identifiers
- DOI
- 10.22002/fta47-ct638
- TILT_SERIES_ID
- wza2017-06-13-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-06-13
- Created
-
2017-07-07
- Updated
-
2017-09-14