Caltech Tomography Archive
Published 2017 | Version v1
Dataset Open

T5_1550Cys_Aumalemaide_5nm

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

This is a sample in which N terminus residue 1550 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.

Technical info

Tilt Series Date: 2017-03-10

Data Taken By: Poorna Subramanian

Species / Specimen: Escherichia coli

Strain: EC93 stick, UPEC536

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 125.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Zach Ruhe and Chris Hayes

Purification / Growth Conditions / Treatment: Freezing in vitrobot. The humidifier was not working. I wouldn't really trust the conditions. See pages 104-105

Sample Preparation: The sample prepared by Taylor Steven

Attached Files

sq7_T5_1693cysAulabelled0001.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

sq7_T5_1693cysAulabelled0001.rec, Reconstruction (Pixel Size 1.56 nm), 890.9 MB

Files

keyimg_psa2017-03-10-1.jpg

Files (4.4 GB)

Name Size
md5:64297ed1ffb358e97cbea9c6d9da0bc3
1.0 MB Preview Download
md5:8ad9f05777cc0d9a0b84fe8ce000ecc8
15.2 MB Preview Download
3.4 GB (external) Download
890.9 MB (external) Download

Additional details

Identifiers

DOI
10.22002/fveqa-hty65
TILT_SERIES_ID
psa2017-03-10-1

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-03-10
Created
2017-03-10
Updated
2019-03-25