Caltech Tomography Archive
Published 2011 | Version v1
Dataset Open

E. coli (pLE7, 20 uM IPTG)

Swulius, Matt1
  • 1. ROR icon California Institute of Technology

Description

Cells contain WT MreB in the chromosome plus an N-terminal YFP fusion to MreB (YFP-MreB) on the plasmid pLe7. pLE7 also confers a resistance to ampicillin.

Files

keyimg_mst2011-06-02-21.jpg
Files (24.9 MB)
Name Size
keymov_mst2011-06-02-21.mp4
md5:35eaa20d47a3e6b148ec95bb26315e4f
23.8 MB Download
keyimg_mst2011-06-02-21.jpg
md5:d702bad5aa6c87a569777329ff3de325
1.1 MB Preview Download

Technical info

Tilt Series Date: 2011-06-02

Data Taken By: Matt Swulius

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: None μm, magnification: 22500x.

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFTomo

Collaborators and Roles: Gift from Larry Rothfield

Purification / Growth Conditions / Treatment: Grown at 37 degrees celsius to OD600=0.2 then 20 uM IPTG was added for 2 hours of growth before freezing.

Sample Preparation: Vitrobot plunge freezing

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mst2011-06-02-21/rawdata/

20110602_PLE7_26.mrc, Tilt Series (Pixel Size 0.95 nm), 1.0 GB Download

20110602_PLE7_26_full.rec, Reconstruction (Pixel Size 1.9 nm), 825.8 MB Download

Additional details

Identifiers Funding Dates
Created:
May 18, 2023
Modified:
August 29, 2025