Published 2016 | Version v1
Dataset Open

NR1769/R1-16 conjugation

Creators

  • 1. ROR icon California Institute of Technology

Description

Grid box YWC231 grid1NR1769/R1-16 conjugating with NR1769 skinny E. coli(mixed for 5 min prior to freezing)

Technical info

Tilt Series Date: 2016-11-16

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain and sample prepared by Carrie Shaffer

ycw2016-11-16-7_slicer17160.jpg: F-pilus machine ycw2016-11-16-7_slicer17161.jpg: F-pilus machine ycw2016-11-16-7_slicer17162.jpg: F-pilus machine ycw2016-11-16-7_slicer17163.jpg: F-pilus machine ycw2016-11-16-7_slicer17164.jpg: F-pilus machine? ycw2016-11-16-7_slicer17165.jpg: F-pilus machine? ycw2016-11-16-7_slicer17166.jpg: F-pilus machine ycw2016-11-16-7_slicer17167.jpg: F-pilus machine ycw2016-11-16-7_slicer17786.jpg: non-piliated T4SS?

Attached Files

20161116_YWC231-1_20007_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB

20161116_YWC231-1_20007.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

Files

keyimg_ycw2016-11-16-7.jpg

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Additional details

Identifiers

DOI
10.22002/g2611-qdy92
TILT_SERIES_ID
ycw2016-11-16-7

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2016-11-16
Created
2016-11-16
Updated
2017-02-14