Myxococcus xanthus delta-pilT
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2015-04-28
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain form Lotte Sogaard-Andersen
Purification / Growth Conditions / Treatment: Cells grown 2 days in 220 rpm shaking CTT medium at 30 degrees without antibiotics. OD600=0.8
Sample Preparation: Vitrobot (MK iv) settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Blot force 6, blot time 2.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200.
Attached Files
20150428_YWC129_10032.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB
20150428_YWC129_10032_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB
Files
keyimg_ycw2015-04-28-31.jpg
Additional details
Identifiers
- DOI
- 10.22002/g5gnz-w8002
- TILT_SERIES_ID
- ycw2015-04-28-31
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-04-28
- Created
-
2015-04-29
- Updated
-
2015-05-11