S. aureus RN6390 ΔESX

Description

Technical info

Tilt Series Date: 2017-09-27

Data Taken By: Georges Chreifi

Species / Specimen: Staphylococcus aureus

Strain: subsp. aureus str. Newman

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2°, constant angular increment, dosage: 265.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: etomo + manual tracking

Collaborators and Roles: Strain provided by Tracy Palmer at the University of Dundee. Data collected and processed by Georges Chreifi

Purification / Growth Conditions / Treatment: Cells were grown in Tryptic Soy Broth (TSB) liquid culture overnight with no supplements. The overnight culture was then used to inoculate fresh TSB, grown until an OD of ~2, and plunge-frozen.

Sample Preparation: 4 grids were frozen. Vitrobot (Mark IV) settings: 95% humidity, 2µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200 mesh. Added 1ul of gold to 4 ul of cells prior to freezing.

Attached Files

atlas_10001.mrc, Tilt Series (Pixel Size 0.39 nm), 363.5 MB

atlas_10001.rec, Reconstruction (Pixel Size 1.56 nm), 222.7 MB

atlas_10009_full.rec, Reconstruction (Pixel Size 1.615 nm), 1.1 GB

atlas_10001_full.rec, Reconstruction (Pixel Size 1.615 nm), 1.1 GB