Sulfolobus cells synchronized for division
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2010-12-03
Data Taken By: Megan Dobro
Species / Specimen: Sulfolobus acidocaldarius
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Ų, defocus: -15.0 μm, magnification: 18000x.
Acquisition Software: Leginon
Upload Method: rundir
Processing Software Used: Raptor
Collaborators and Roles: grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University
Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine
Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity
mdj2010-12-03-9_slicer3751.jpg: archellum
Attached Files
10dec03f_Box13grid1_004new.mrc, Tilt Series (Pixel Size 1.258 nm), 1.1 GB
10dec03f_Box13grid1_004new_full.rec, Reconstruction (Pixel Size 2.38 nm), 1.0 GB
Files
keyimg_mdj2010-12-03-9.jpg
Additional details
Identifiers
- DOI
- 10.22002/g9v2d-44404
- TILT_SERIES_ID
- mdj2010-12-03-9
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2010-12-03
- Created
-
2012-07-09
- Updated
-
2014-03-12