Myxococcus xanthus delta-pilT

Description

Technical info

Tilt Series Date: 2013-12-21

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -4 offset, 4 sec blot time, 1s drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder. 10 nm gold beads.

ycw2013-12-21-65_slicer16272.jpg: double leaflets ycw2013-12-21-65_slicer16271.jpg: Double leaflets ycw2013-12-21-65_slicer16270.jpg: Double leaflets ycw2013-12-21-65_slicer16268.jpg: double leaflets of IM & OM ycw2013-12-21-65_slicer16267.jpg: double leaflets of IM & OM

Attached Files

20131221_YWC063_20022.mrc, Tilt Series (Pixel Size 0.39 nm), 3.6 GB

20131221_YWC063_20022_part126_2.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB