Myxococcus xanthus JK328
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-07-22
Data Taken By: Yi-Wei Chang
Species / Specimen: Myxococcus xanthus
Strain:
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -10.0 μm, magnification: 26000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Martin Thanbichler
Purification / Growth Conditions / Treatment: Cells grown 48 hr in CTT medium at 30 degrees without antibiotics. OD600=0.7
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200
ycw2013-07-22-76_slicer9115.jpg: filament bundle, Myxococcus Xanthus
Attached Files
07222013_Janelia_Myxo_JK328_20018.mrc, Tilt Series (Pixel Size 0.42 nm), 3.6 GB
07222013_Janelia_Myxo_JK328_20018_part131_8.rec, Reconstruction (Pixel Size 1.68 nm), 430.6 MB
Files
keyimg_ycw2013-07-22-76.jpg
Additional details
Identifiers
- DOI
- 10.22002/ggqj4-kap12
- TILT_SERIES_ID
- ycw2013-07-22-76
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-07-22
- Created
-
2013-07-24
- Updated
-
2014-07-31