Caltech Tomography Archive
Published 2020 | Version v1
Dataset Open

D. flos-aquae Gas Vesicles

Dutka, Przemek1
  • 1. ROR icon California Institute of Technology

Description

Gas vesicles purified from D. flos-aquae, WT

Files

keyimg_pda2020-11-04-6.jpg
Files (11.2 MB)
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keyimg_pda2020-11-04-6.jpg
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Technical info

Tilt Series Date: 2020-11-04

Data Taken By: Przemek Dutka

Species / Specimen: Dolichospermum flos-aquae

Strain: CCAP 1403/13F

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 50.0 eV/Ų, defocus: 2.5 μm, magnification: 64000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used:

Collaborators and Roles: purified by Dina Malounda the Shapiro lab; data collected by Przemek . Dutka and Songye Chen

Purification / Growth Conditions / Treatment: Lakshmanan, A., Lu, G., Farhadi, A. et al. Preparation of biogenic gas . vesicle nanostructures for use as contrast agents for ultrasound and . MRI. Nat Protoc 12, 2050–2080 (2017). . https://doi.org/10.1038/nprot.2017.081. Sample in 10mM Hepes, pH7.4; OD 5.0

Sample Preparation: Quantifoil C2/2 200mesh extra thick carbon, 10nm BSA-gold, 100% . humidity, Blot time 4, Blot force 3, temp 4C

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/pda2020-11-04-6/rawdata/

AnaGVOD5_5_movies.mrc, Tilt Series (Pixel Size 0.14 nm), 1.9 GB Download

AnaGVOD5_5.mrc.mdoc, 26.9 kB Download

Additional details

Identifiers Funding Dates
Created:
June 6, 2023
Modified:
August 29, 2025