Published 2010
| Version v1
Dataset
Open
HUVECs CHMP4A+VPS4DN
Description
HUVECs transfected with CHMP4A+VPS4DN constructs using Nucleofector Kit. Sample# C58-2
Technical info
Tilt Series Date: 2010-12-13
Data Taken By: Cora Woodward
Species / Specimen: HUVECs
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Acquisition Software: Leginon
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: CHMP4A and VPS4DN construct provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected
Sample Preparation: manual blotting
Attached Files
10dec13a_C45_1_013_full.rec, Reconstruction (Pixel Size 2.0 nm), 2.7 GB
10dec13a_C45_1_013.st, Tilt Series (Pixel Size 0.5 nm), 1.0 GB
Files
keyimg_cwl2010-12-13-10.jpg
Additional details
Identifiers
- DOI
- 10.22002/gjmqv-vtk65
- TILT_SERIES_ID
- cwl2010-12-13-10
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2010-12-13
- Created
-
2015-09-16
- Updated
-
2015-09-16